规格
|
Exonuclease Activity:
|
3' - 5', 5' - 3' |
Fidelity (vs. Taq):
|
6 X |
GC-Rich PCR Performance:
|
Low |
Hot Start:
|
Built-In Hot Start |
Overhang:
|
Mixed |
Polymerase:
|
Platinum® Taq DNA Polymerase High Fidelity |
Product Line:
|
Platinum® |
Quantity:
|
5000 reactions |
Reaction Format:
|
SuperMix or Master Mix |
Reaction Speed:
|
Standard |
Shipping Condition:
|
Approved for shipment on Wet or Dry Ice |
Size (Final Product):
|
15 kb or less |
储存
|
• 4 × 56.25 mL Platinum PCR SuperMix High Fidelity
Store at -20°C in a non-frost-free freezer. |
Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:
• Achieve greater than six-times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 15 kb
• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs
• Minimize PCR optimization
Using Platinum® PCR SuperMix, High Fidelity
Platinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic 'hot-start' PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.