规格
Detection Method:	Biotin-Based
Final Product Type:	cDNA (Double-Stranded)
Sample Type:	Poly(A+) RNA, Total RNA
Includes Label or Dye:	No
Label or Dye:	Biotin
Labeling Method:	Direct Labeling
Labeling Target:	cDNA
Remarks:	For generating double-stranded cDNA
Shipping Condition:	Dry Ice
System Type:	Affymetrix GeneChip®
Product Line:	SuperScript™

储存
50 µM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript® II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water All components are guaranteed stable for 6 months when properly stored.

描述

The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript® II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).

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