REPLI-g Midi Kit (100)，150045，Qiagen，凯杰

Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). REPLI‑g Kits provide DNA polymerase, buffers and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue and cells. QIAGEN REPLI-g Kits are available in several different sizes and configurations which allows researchers to build the best workflow for their application and lab space. Typical REPLI-g yields range from approx. 7 µg to 40 µg of amplified DNA, depending on which kit and workflow is used. The REPLI-g Mini and Midi Kits offer a single tube workflow with higher yields of amplified DNA, while the REPLI-g UltraFast Kit enables highly uniform and accurate whole genome amplification in just 60–90 minutes. For high-throughput processing in 96-well format, the streamlined, room-temperature procedure of the REPLI-g Screening Kit enables straightforward liquid handling that requires just 20 minutes. The REPLI-g Screening kit is intended for rapid screening using a range of genotyping, PCR, sequencing or microarray assays.

• Amplified DNA can be used in NGS, microarray and PCR applications
• Isothermal multiple displacement amplification (MDA) with proof-reading DNA polymerase
• Whole genome amplification (WGA) yields 7 to 40 µg amplified DNA from 10 ng of DNA
• Ultrafast format for whole genome amplification in 1–1.5 hours
• High-throughput format for 96 samples with just 20 minutes of handling time

• REPLI-g amplified genomic can be used in a variety of downstream applications, including:
• SNP genotyping with TaqMan^® primer/probe sets
• qPCR- and PCR-based mutation detection
• Next-generation sequencing
• STR/microsatellite analysis
• Sanger sequencing
• RFLP and Southern blot analysis
• Array technologies, such as comparative genomic hybridization