QIAGEN Multiplex PCR Kit (100)，206143，Qiagen，凯杰

For 100 x 50 µl multiplex PCR reactions: 2x QIAGEN Multiplex PCR Master Mix (providing a final concentration of 3 mM MgCl2, 3 x 0.85 ml), 5x Q-Solution (1 x 2.0 ml), RNase-Free Water (2 x 1.7 ml)

用于高特异性和灵敏度的多重PCR，无需优化

无需优化步骤

高特异性和高灵敏度，热启动

高度适合用于各种类型的多重PCR应用

使用简单，经济实惠

QIAGEN Multiplex PCR Kit提供方便的即用型预混液。QIAGEN Multiplex PCR Master Mix包含HotStarTaq DNA Polymerase和含有新型合成因子MP的独特PCR缓冲液。配合优化的盐浓度，MP因子可以促进引物与模板的特异性结合，使反应体系中所有的引物都能有效延伸，无需额外优化。该试剂盒还包含新型的辅助剂Q-Solution，有助于实现“困难”模板（比如，GC含量高的模板）的高效扩增。

QIAGEN Multiplex PCR Kit可确保高特异性和灵敏度的多重PCR扩增。该试剂盒能成功应用于各种多重应用，如转基因生物分型和微卫星分析。该混合物包含HotStarTaq DNA Polymerase，用于高效的平行扩增多个靶。扩增效率经新型的PCR缓冲液进一步优化，该缓冲液也包含在混合物中。独特的缓冲液确保了在广泛的PCR条件下PCR的特异性，无需优化步骤。试剂盒还包含辅助剂Q-Solution，用于扩增高GC含量的模板，优化PCR反应。

HotStarTaq DNA Polymerase规格

浓度：5单位/µl
重组酶：有
底物类似物：dNTP、ddNTP、dUTP、biotin-11-dUTP、DIG-11-dUTP、荧光-dNTP/ddNTP
延伸率：72°C下2–4 kb/分钟
半衰期：97°C下10分钟；94°C下60分钟
扩增效率：≥105倍
5'–>3'外切酶活性：有
Extra A辅助剂: 有
3'–>5'外切酶活性：无
污染核酸：无
污染RNA酶：无
污染蛋白酶：无
自吸泵活性：无

Successful microsatellite analysis.

Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and Penta E was carried out using 1 ng of K562 human genomic DNA and fluorescein-labeled primers. Reactions were analyzed on the ABI PRISM 377 Sequencer. Top: The QIAGEN Multiplex PCR Kit ensured high sensitivity and uniform signal intensity. Bottom: Results using a hot-start DNA polymerase from Supplier AII.

Stable and efficient primer annealing.

Genotyping transgenic mice.

Transgenic mice were screened using the QIAGEN Multiplex PCR Kit and sets of 3 primers to distinguish wild-type (wt), heterozygous mutant (ht), and homozygous mutant (hm) mice. [A] Using a primer set for the recombination activating gene 2 locus. [B] Using a primer set for the interferon-γ gene locus. M: markers. (Data kindly provided by S. zur Lage and S. Weiss, National Research Center for Biotechnology, Braunschweig, Germany.)

Successful 16-plex PCR.

Multiplex PCR of 16 targets (99–955 bp) was carried out for 35 cycles using standard conditions for the QIAGEN Multiplex PCR Kit, without further optimization or using a variety of conditions with a hot-start DNA polymerase from Supplier AII. [A] Comparison using 2.5 units per 50 µl reaction of the hot-start DNA polymerase from Supplier AII and with the indicated Mg2+ concentrations. [B] Comparison using the optimized Mg2+ concentration (3.5 mM) for the hot-start DNA polymerase from Supplier AII (part A) and the indicated amounts of enzyme per 50 µl reaction. Successful results were ensured with the QIAGEN Multiplex PCR Kit. M: markers.

QIAGEN Multiplex PCR Kit高度适用于各种多重应用，包括：

转基因生物的分型和分析

微卫星的扩增和分析

细菌和病毒的分型和检测

用于SNP分析的多个DNA扩增