规格
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Sample Type:
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Synthetic siRNA, miRNA |
Control Type:
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Negative Control, Gene Knockdown Positive Control |
Transfection Technique:
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Lipid-Based Transfection |
Product Line:
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Silencer™, Ambion™ |
储存
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siPORT™ NeoFX™ Transfection Agent and siPORT™ Amine Transfection Agent should be stored at 4°C. GAPDH siRNA and Negative Control siRNA should be stored at –20°C. |
This Ambion® kit is for the optimization of transfection protocols for specific cell types. It includes 0.4 mL of each of two transfection agents; siPORT™ Amine Transfection Agent and siPORT™ NeoFX™ Transfection Agent along with control siRNAs.
• Broad cell line compatibility-kit includes siPORT™ Amine and siPORT™ NeoFX™ transfection agents
• Faster protocol optimization-kit includes ready-to-transfect GAPDH and negative siRNA controls The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, the Silencer® siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. The Silencer® siRNA Transfection II Kit includes siPORT™ Amine Transfection Agent (a polyamine mixture) and the new siPORT™ NeoFX™ Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types. Furthermore, both can be used to transfect cells during subculturing-saving a full day of valuable time-without increased cytotoxicity. In addition to containing a choice of transfection agents, this kit includes a well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies. A scrambled, negative control siRNA is also provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.