规格
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Assay Entry:
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Biochemical terbium phospho-specific activity |
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Detection Method:
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Fluorescent |
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Label or Dye:
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GFP (EmGFP) |
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Shipping Condition:
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Dry Ice |
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Substrate:
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4E-BP1 |
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Substrate Properties:
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Protein-Based Substrate |
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Target Entry:
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mTOR, FRAP1 |
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Target Enzyme:
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Serine⁄Threonine Kinases |
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Validated Kinases:
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FRAP1 (mTOR), FRAP1 (mTOR) |
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Substrate Type:
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Kinase Substrate |
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Molecular Weight:
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45600 Da |
储存
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GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2mM DTT. Store at -80°C. |
4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
For use in combination with the LanthaScreen® Tb-anti-4E-BP1 [pThr46] Antibody (PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.