QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 μl up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1–12 samples on the QIAcube Connect.
QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure " Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure " Paternity testing by RFLP analysis").
The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery of DNA; the QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table “DNA yields from human whole blood with different cell densities”).
DNA yields from human whole blood with different cell densities
Leukocytes per ml | DNA yield (µg) | DNA recovery (%) |
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| QIAamp Midi | QIAamp Maxi | QIAamp Midi | QIAamp Maxi |
2.5 x 105 | 3.0 | 15.8 | 92.3 | 95.8 |
1.0 x 106 | 11.0 | 60.4 | 91.7 | 91.5 |
5.0 x 106 | 62.4 | 312.0 | 94.5 | 94.5 |
1.0 x 107 | 116.3 | 624.6 | 88.1 | 94.6 |