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General description
Vacuolar protein sorting-associated protein 72 homolog (UniProt: Q15906; also known as Protein YL-1, Transcription factor-like 1) is encoded by the VPS72 (also known as TCFL1, YL1) gene (Gene ID: 6944) in human. YL-1 is a nuclear protein that may serve as a DNA-binding transcriptional regulator and may be involved in chromatin modification and remodeling. YL-1 is plays multiple roles in chromatin modification and remodeling in cells and it is shown to be a subunit of TRRAP/TIP60 HAT complex and of chromatin remodeling SCCAP-containing complex. It has a DNA binding region that includes amino acids 156 to 206. In addition, it has an aspartic/glutamic acid rich acidic region (aa 22-77) and a proline rich region (aa 333-354). Two isoforms of YL-1 have been described that are produced by alternative splicing. (Ref.: Cai, Y., et al. (2006). J. Biol. Chem. 280 (14); 13665-70).
~48 kDa observed, 40.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST-tagged full-length recombinant human YL-1 protein.
Application
Anti-YL-1, clone EX-1Y32, Cat. No. MABE1796, is a highly specific mouse monoclonal antibody that targets Vacuolar protein sorting-associated protein 72 homolog and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected YL-1 in Western Blotting applications (Obri, A., et. al. (2014). Nature. 505(7485):648-53).
Immunocytochemistry Analysis: A 1:800 dilution from a representative lot detected YL-1 in HeLa cells.
Western Blotting Analysis: 2 µg/mL from a representative lot detected YL-1 in 10 µg of HeLa cell lysate.
Immunoprecipitation Analysis: 1-10 µg from a representative lot detected YL-1 in Mouse embryonic fibroblast (MEF) total cell extracts.
Biochem/physiol Actions
Clone EX-1Y32 detects Vacuolar protein sorting-associated protein 72 homolog (YL-1) in human and murine cells.
Physical form
Format: Purified
Protein A + Protein G
Purified mouse monoclonal antibody IgG1 in PBS without azide and 40% glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Mouse embryonic fibroblast (MEF) cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected YL-1 in Mouse embryonic fibroblast (MEF) cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | EX-1Y32, monoclonal |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_005988 |
| UniProt accession no. | Q15906 |
| target post-translational modification | unmodified |
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