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Anti-Kaiso Antibody, clone 6F，200UG，05-659，Sigma

销售价： ¥ 3747.78 / 件

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订货号： 0BW1715

品牌型号： Sigma 05-659

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最小订货量： 1件

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商品介绍
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包装参数

产品介绍 Product Description

General description

97kDa

Immunogen

Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso

Application

Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Biochem/physiol Actions

Kaiso

Physical form

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
Format: Purified
Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	purified antibody
antibody product type	primary antibodies
clone	6F, monoclonal
species reactivity	mouse, hamster, chicken, canine, rat, human, monkey
manufacturer/tradename	Upstate^®
technique(s)	electrophoretic mobility shift assay: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable
isotype	IgG1
NCBI accession no.	NM_006777
UniProt accession no.	Q86T24
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	human ... ZBTB33(10009)

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