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General description
Chromobox protein homolog 5, also known as Antigen p25, Heterochromatin protein 1 homolog alpha (HP1 alpha), and encoded by the gene name CBX5 or HP1A is a component of heterochromatin that recognizes and binds histone H3 tails methylated at ′Lys-9′ (H3K9me), leading to epigenetic repression. In contrast, Chromobox protein homolog 5 is excluded from chromatin when ′Tyr-41′ of histone H3 (H3Y41ph) is phosphorylated. CBX5 is implied to interact with lamin-B receptor (LBR), which can contribute to the association of the heterochromatin with the inner nuclear membrane. Recent studies have shown that CBX5 is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
~23 kDa observed
Immunogen
BCP-conjugated linear peptide corresponding to human Chromobox protein homolog 5.
Application
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Chromobox protein homolog 5 in HeLa and A431 cells.
Alexa Fluor™ is a registered trademark of Life Technologies
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Chromobox protein homolog 5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Chromobox protein homolog 5.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Chromobox protein homolog 5 in 10 µg of HeLa nuclear extract.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | gorilla (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), giant panda (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_001120793 |
| UniProt accession no. | P45973 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CBX5(23468) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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