- 商品介绍
- 规格参数
- 包装参数
General description
The sarcoplasmic reticulum of striated muscle is a specialized intracellular membrane system that plays a critical role in the contraction and relaxation of muscle. The sarco/endoplasmic reticulum Ca2+ ATPases (SERCAs) mediate Ca2+ uptake into intracellular stores. SERCA mediated Ca2+ uptake induces and maintains muscular relaxation. SERCA1 is expressed in fast-twitch muscles, whereas SERCA2 is expressed in cardiac and slow-twitch muscles. SERCA2 plays an important part in regulating cardiac contractile function.
~ 110 kDa
Immunogen
Epitope: Unknown
Purified canine cardiac sarcoplasmic reticulum.
Application
Anti-SERCA2 Antibody, clone IID8 is an antibody against SERCA2 for use in WB, IC, IH.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Eizema, 2007).
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeletal Signaling
Biochem/physiol Actions
The antibody recognizes SERCA2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of the antibody detected SERCA2 in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | IID8, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001003214 |
| UniProt accession no. | O46674 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ATP2A2(488) |
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