Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4,100UG,MAB3484,Sigma

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订货号 0BW1545
品牌型号 Sigma MAB3484
货期 询货期
最小订货量 1件
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产品介绍 Product Description

Immunogen

Epitope: a.a. 386-412 of human CFTR


Application

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4 is an antibody against Cystic Fibrosis Transmembrane Conductance Regulator for use in IC, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-10μg/mL, note do not boil the lysate, incubate at 80C for 30 minutes prior to running SDS-PAGE. CTFR aggregates upon boiling. Antibody recognizes CFTR at 170kDa and two additional proteins at 100 & 140kDa.

Immunoprecipitation

Immunofluorescence

Note: Does not work on paraffin embedded tissue.

Optimal working dilutions must be determined by the end user.


Biochem/physiol Actions

Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the cytoplasmic region preceding the first nucleotide binding domain between residues 386 to 412.

Recognizes CFTR, Mr 170kDa and two additional proteins at ~ Mr 100 and 140kDa.


Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.


Preparation Note

Maintain at 2-8°C in undiluted aliquots up to 6 months.


Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcemouse
Quality Segment100
conjugateunconjugated
antibody formpurified immunoglobulin
antibody product typeprimary antibodies
cloneL12B4, monoclonal
species reactivityhuman
should not react with shark, mouse
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable
isotypeIgG2a
NCBI accession no.NM_000492.3
UniProt accession no.P13569
shipped inwet ice
target post-translational modificationunmodified
Gene Informationhuman ... CFTR(1080)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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