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General description
FAT10 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. FAT10, a ubiquitin-like protein, is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. FAT10 expression causes apoptosis and it appears that FAT10 may modulate tumorigenesis through its interaction with the MAD2 spindle-assembly checkpoint protein. It has been demonstrated that FAT10 and its conjugates are rapidly degraded by the proteasome. Furthermore, a new interaction partner of FAT10, NEDD8 ultimate buster-1L (NUB1L), may function as a linker that targets FAT10 for degradation by the proteasome.
~18 kDa observed. FAT10 FAT10ylates itself as well as other proteins so additional bands may be observed due to the FAT10ylation of endogenous proteins (Aichem, A., et al. (2010). Nature Commun. 1:13.),(Aichem, A., et al. J Cell Sci. (2012)125:4576-85).
Immunogen
GST-tagged recombinant protein corresponding to human FAT10.
Application
Anti-FAT10 Antibody, clone 4F1 is a highly specific mouse monoclonal antibody, that targets FAT10 & has been tested in western blotting, IP & ICC.
Immunocytochemistry Analysis: A representative lot detected FAT10 in HA-FAT10 transfected HEK293 cells (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4F1, monoclonal |
| species reactivity | rat, mouse, human |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NP_006389 |
| UniProt accession no. | O15205 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UBD(10537) |
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¥ 1724.25
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¥ 9737.84
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¥ 2573.97
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¥ 3335.66
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¥ 4011.97
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