Anti-Calreticulin Mouse mAb (FMC75)，50UG，208912-50UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.

Immunogen

Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein

Application

Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% Glycerol and 0.09% Sodium Azide.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

Other Notes

Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	FMC75, monoclonal
form	liquid
does not contain	preservative
species reactivity	human, monkey
should not react with	rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
dilution	(Enzyme Immunoassay (0.1 µg/mL) Immunoblotting (0.5 µg/mL) Immunocytochemistry Immunohistochemistry Flow Cytometry )
isotype	IgG1
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified

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