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General description
CYLD is a deubiquitinating enzyme that inactivates transcription factor NF-kappaB using TNFRs and is essential for normal cellular homeostasis of skin appendages. Deactivation or mutation of CYLD causes a loss of the deubiquitination activity and increases tumorigenesis. Loss of CYLD specifically results in familial cylindromatosis which is characterized by a predisposition to cylindromas, tumors of skin appendages. CYLD has been shown to cleave the lysine 63-linked polyubiquitin chain from proteins and the inactivity of CYLD has also been noticed in many other human cancers.
~110 kDa observed.
Uncharacterized band(s) may appear in some lysates.
Immunogen
Linear peptide corresponding to human phospho-CYLD (Ser418).
Application
This Anti-phospho-CYLD Antibody (Ser418) is validated for use in Western Blotting for the detection of phospho-CYLD (Ser418).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-CYLD (Ser418) in 10 µg of human spleen and Jurkat lysate.
Western Blotting Analysis: A representative lot detected phospho-CYLD (Ser418) in PMA and Ionomycin-treated Jurkat cell lysate (Reiley, W., et al. (2005). Mole. Cell Biol. 25(10):3886-3895).
Biochem/physiol Actions
This antibody detects phospho-CYLD at Ser418. The phosphorylation site of CYLD shares some sequence homology with serine phosphorylation site of c-Cbl.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-CYLD (Ser418) in 10 µg of Raji cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_001035814 |
| UniProt accession no. | Q9NQC7 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer418) |
| Gene Information | human ... CYLD(1540) |
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