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- 规格参数
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General description
Ras related protein Rab-10 (Rab10) is encoded by the gene named RAB10 and is member of the small GTPase family of Rab proteins. Rab proteins play critical roles in intracellular trafficking from the formation of vesicles to their fusion with membranes. Specifically Rab10 is involved in regulating RME-1 in endocytic recycling as well as endoplasmic reticulum structural dynamics and morphology, and Rab10 also appears to be critical to formation and correct placement of proteins in such critical cellular structures as the basement membranes of during organ morphogenesis and basement membrane remodeling during development. Rab10 localizes to internal membrane structures like the endoplasmic reticulum and Golgi apparatus but in certain cells it can be found associated with cilia and other cellular structures as well. EMD-Millipore’s Anti-Rab10 mouse monoclonal antibody has been tested in western blot against human HeLa and mouse NIH3T3 cell lysates and in fluorescent immunocytochemistry on LOVO cells in culture.
~25 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen
Purified recombinant fragment of human Rab10 expressed in E. Coli.
Application
Detect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Rab10 in LOVO cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa and NIH/3T3 cell lysates
Evaluated by Western Blotting in HeLa and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 4E2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunofluorescence: suitable,western blot: suitable |
| isotype | IgG1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RAB10(10890) |
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¥ 1596.77
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¥ 1259.40
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¥ 4897.21
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¥ 14407.09
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¥ 2666.57
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¥ 1476.00
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¥ 2452.91
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¥ 2911.61
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¥ 5426.00
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