Anti-MSH2 (Ab-1) Mouse mAb (GB12)，100UG，NA26-100UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20 mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and colon tissue.
This Anti-MSH2 (Ab-1) Mouse mAb (GB12) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of MSH2 (Ab-1).

Immunogen

Human
an N-terminal fragment of human MSH2

Application

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunoprecipitation (1 µg/reaction)

Paraffin Sections (3 µg/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
LoVo cells
Positive Control
HCT116 or SW480 cells or colon tissue

Other Notes

Bronner, C. E., et al. 1994. Nature368, 258.
Papadopoulos, N., et al. 1994. Science263, 1625.
Peltomäki, P. T. 1994. Annals of Medicine26, 215.
Fishel, R., et al. 1993. Cell75, 1027-1038, 1993.
Leach, F. S., et al. 1993. Cell75, 1215.
Lindbolm, A., et al. 1993. Nature Genetics5, 279.
For paraffin sections, pre-treat with heat for 20 min. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	GB12, monoclonal
form	liquid
contains	≤0.1% sodium azide as preservative
species reactivity	human
should not react with	mouse
manufacturer/tradename	Calbiochem^®
storage condition	do not freeze
dilution	(Frozen Sections (5 µg/mL) Immunoblotting (1 µg/mL) Immunoprecipitation (1 µg/reaction) Paraffin Sections (3 µg/mL, heat pre-treatment required))
isotype	IgG1
shipped in	wet ice
storage temp.	2-8°C
target post-translational modification	unmodified
Gene Information	human ... MSH2(4436)

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