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General description
Methyl-CpG-binding domain protein 5 (UniProt: Q9P267; also known as Methyl-CpG-binding protein MBD5) is encoded by the MBD5 (also known as KIAA1461) gene (Gene ID: 55777) in human. MBD proteins play a role in diverse processes, including chromatin remodeling, DNA damage repair, histone methylation, and X chromosome inactivation. MBD5 is detected in heart, placenta, liver, skeletal muscle, kidney, and pancreas. It binds to heterochromatin, but does not interact with either methylated or unmethylated DNA (in vitro). It contains a MBD domain (aa 11-81) and a PWWP (Pro-Trp-Trp-Pro) domain (aa 1385-1447). Both MBD and PWWP domains are shown to be essential for chromocentric localization. Two isoforms of MBD5 have been reported that are produced by alternative splicing. However, only the isoform 1 associates with pericentric heterochromatin. Mutations in MBD5 gene are known to cause autosomal dominant mental retardation, which is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. (Ref.: Camarena, V et al., (2014). EMBO Mol. Med. 6(8); 1003 1015).
~160 kDa observed; 159.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: unknown
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Methyl-CpG-binding domain protein 5 (MBD5).
Application
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD5 in human pancreas and human cervical cancer tissue sections
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD5 in HeLa, A431, and HEK293 cells.
Western Blotting Analysis: 2 µg/mL from a representative lot detected MBD5 in human liver tissue lysate.
Anti-MBD5, Cat. No. ABE1322, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
This rabbit polyclonal antibody detects isoform 1 of human Methyl-CpG-binding domain protein 5. It targets an epitope within 16 amino acids fromt the C-terminal region.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry in human prostate tissue sections.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MBD5 in human prostate tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_060798.2 |
| UniProt accession no. | Q9P267 |
| target post-translational modification | unmodified |
| Gene Information | human ... MBD5(55777) |
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