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General description
Spartin is also known as Spastic paraplegia 20 protein and Trans-activated by hepatitis C virus core protein 1. Spartin has been implicated in endosomal trafficking and microtubule dynamics. Spartin is ubiquitously expressed, but the highest expression found in adipose tissue. Aberrant spartin can cause spastic paraplegia autosomal recessive type 20 (SPG20).
~78 kDa observed
Immunogen
Recombinant protein corresponding to human Spartin.
Application
Detect Spartin using this rabbit polyclonal antibody, Anti-Spartin Antibody validated for use in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Spartin in 10 µg of mouse brain tissue lysate and PC12 cell lysate.
Western Blotting Analysis: A representative lot detected Spartin in HeLa, NSC34, and PC12 cell lysates (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Spartin in HeLa cell lysate (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).
Immunofluorescence Analyis: A representative lot detected Spartin in HeLa cells (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HEK293 cell lysate
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Spartin in 10 µg of HEK293 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_001135766 |
| UniProt accession no. | Q8N0X7 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CUX1(1523) |
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¥ 1082.93
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¥ 1524.91
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¥ 1761.75
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¥ 713.29
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¥ 4274.19
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¥ 66743.99
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¥ 64659.66
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