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General description
Epithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.
~ 40 kDa
Immunogen
Epitope: Unknown
HES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)
Application
Flow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected EpCAM in human liver tissue, iPSC-derived liver organoid progenitor, and mature liver organoid sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected EpCAM in human breast tissue and patient-derived triple negative breast cancer organoid sections.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected EpCAM in human small intestine, human colon, duodenum intestinal organoid and colon intestinal organoid tissue sections.
Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Adhesion (CAMs)
This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).
Biochem/physiol Actions
This antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human embryonic stem cells
Evaluated by Immunocytochemistry in human embryonic stem cells.
Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| clone | mab 8, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunofluorescence: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_002345.2 |
| UniProt accession no. | P16422 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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