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- 规格参数
- 包装参数
General description
The exocyst complex component 70 (Exo70) is a component of the exocyst complex, an octameric structure that is also comprised of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. The exocyst complex facilitates the fusion of secretory vesicles with the plasma membrane and the release of vesicular cargo, which may include neurotransmitters, hormones, and membrane proteins. Previous studies have indicated that Exo70 plays an important role in actin assembly and cell motility.
~75 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to rat Exo70.
Application
Detect Exo70 using this Anti-Exo70 Antibody, clone 70X13F3 validated for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected Exo70 in 10 µg of human brain tissue lysate.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in Exo70 cells. (Hsu, S.C., et al. (2001). The Journal of Neuroscience. 21(11):3839–3848.)
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hsu, S.C., et al. (2011). The Journal of Neuroscience. 31(10):3522–3535.)
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Exo70 in 10 µg of rat brain tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 70X13F3, monoclonal |
| species reactivity | human, rat |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_073182 |
| UniProt accession no. | O54922 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... EXOC7(23265) |
| 长度(mm) | |
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