- 商品介绍
- 规格参数
- 包装参数
Immunogen
Epitope: cellular
Purified fibronectin from Human A8387 fibrosarcoma cells.
Application
Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.
Immunoblotting at 1:1,000
Indirect immunohistochemistry at 1:200-1:400
A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).
Final working dilutions must be determined by end user.
Biochem/physiol Actions
Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.
Physical form
Format: Purified
In PBS with 1% BSA and 0.1% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | DH1, monoclonal |
| species reactivity | rat, guinea pig, human, rabbit |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_002026.2,NM_054034.2,NM_212474.1,NM_212475.1,NM_212476.1,NM_212478.1,NM_212482.1 |
| UniProt accession no. | P02751 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FN1(2335) rabbit ... Fn1(100328589) rat ... Fn1(25661) |
| 长度(mm) | |
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| 重量(kg) |
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