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General description
Histidine-tagged full-length mature part of bovine PrP (25-244) is expressed in E. Coli BL21, solubilized from inclusion bodies in 6 M guanidine-HCl, and purified by Ni(II)-nitriloacetate agarose chromatography followed by reversed-phase HPLC (C4 column)
Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner 1998). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPSc) (Pan et al. 1993). Both PrPSc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids (Basler et al. 1986). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope et al. 1986; Turk et al. 1988) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor (Stahl et al. 1987, 1990). The mature protein of 209 amino acids contains one disulfide bond (Turk et al. 1988) and has two sites of asparagine-linked glycosylation (Endo et al. 1989; Oesch et al. 1995).
Application
Antigen in standard immunochemical detection of BSE.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Physical form
Liquid in 10 mM sodium acetate buffer, pH 4.0, containing 0.01% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
The PrPc appears as a single band of about 27 kD by SDS-PAGE
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | bovine |
| Quality Segment | 100 |
| assay | >95% (total protein) |
| form | liquid |
| manufacturer/tradename | Chemicon® |
| technique(s) | cell based assay: suitable |
| NCBI accession no. | NM_000311.3,NM_001080121.1,NM_001080122.1,NM_001080123.1,NM_183079.2 |
| UniProt accession no. | P04156 |
| shipped in | dry ice |
| Gene Information | bovine (calf) ... PRNP(281427) |
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