Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)，100UG，GR15-100UGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

Immunogen

Human
partially purified, human EGFR from A431 cells

Application

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
HL-60 cells
Positive Control
A-431 cells

Other Notes

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	EGFR.1, monoclonal
form	liquid
contains	≤0.1% sodium azide as preservative
species reactivity	human
should not react with	rat, mouse
manufacturer/tradename	Calbiochem^®
storage condition	do not freeze
dilution	(Flow Cytometry (1-5 µg/mL) Immunofluorescence (1-5 µg/mL) Immunoprecipitation (1-2 µg/sample) Neutralization Studies (1-2 µg/mL) )
isotype	IgG2b
shipped in	wet ice
storage temp.	2-8°C
target post-translational modification	unmodified
Gene Information	human ... EGFR(1956) mouse ... Egfr(13649) rat ... Egf(25313)

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