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General description
Retinol-binding protein 3 (UniProt: P12661; also known as Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3, Protein 7S) is encoded by the RBP3 gene (Gene ID: 281443) in bovine. RBP3 is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-22) that are subsequently cleaved off to produce the mature form. It is a lipophilic glycoprotein that is secreted by rod and cone photoreceptors and plays a major role in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Its expression is observed early in retinal differentiation. RBP3 shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. It can bind many retinoids and polyunsaturated fatty acids and in addition to retinoid binding, it also plays a role in retinal development and homeostasis. RBP3 also displays thiol-dependent antioxidant activity that maintains the delicate redox balance in the normal retina. RBP3 dysregulation has been linked to retinal diseases. Mice lacking RBP3 exhibit severe early and progressive photoreceptor degeneration that is characterized by a reduction in both length and numbers of cone sheaths. In mammals four protein subunits of RBP3 have been described (M1-4), each consisting of about 300 amino acids. Clone mAb5 has been mapped to M2 subunit of RBP3. (Ref.: Sears, AE., et al. (2020) FASEB J. 34(10); 13918-13934; Zheng, S., et al. (2020). Front. Cell. Neurosci. 14; Article 577935).
Immunogen
Purified Retinol-binding protein 3 (RBP3) from bovine retina.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from Bovine retinal tissue.
Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.
Tested Applications
Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone mAb5 is a mouse monoclonal antibody that detects Retinol-binding protein 3 (RBP3). It targets an epitope within the N-terminal half.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | mAb5, monoclonal |
| species reactivity | bovine |
| packaging | antibody small pack of 100 |
| technique(s) | electron microscopy: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_776589 |
| UniProt accession no. | P12661 |
| target post-translational modification | unmodified |
| Gene Information | bovine ... RBP3(281443) |
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