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General description
Phosphatidylcholine-sterol acyltransferase (EC 2.3.1.43; UniProt P16301; also known as Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT) is encoded by the Lcat (also known as AI046659; D8Wsu61e) gene (Gene ID 16816) in murine species. LCAT catalyzes the esterification of cholesterol and phosphatidylcholines (PCs) to cholesteryl esters and lysoPCs on the surface of high and low density lipoproteins (HDLs and LDLs). LCAT reaction is essential for the maturation of nascent HDL particles in plasma, and a genetic deficiency of LCAT results in the near absence of normal HDL particles and the presence of plasma very low density and low density lipoprotein particles of abnormal size and shape. LCAT is initially produced with a 24-aa N-terminal signal peptide, which is later cleaved to form the mature enzyme. LCAT is synthesized in the liver before it is secreted into the plasma.
~60 kDa observed. Target band size appears larger than the calculated molecular weight (49.7 kDa/preform & 49.0 kDa/mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Immunogen
Recombinant protein corresponding to mouse Lcat.
Application
This Anti-Lcat Antibody is validated for use in Western Blotting for the detection of Lcat .
Western Blotting Analysis: A representative lot detected Lcat in FPLC-fractionated, TCA-precipitated plasma HDL from wild-type mice as well as mice with human apoM and/or A-I transgene (Liu, M., et al. (2014). J Biol Chem. 289(5)2801-2814).
Western Blotting Analysis: A representative lot detected Lcat in plasma from WT and endothelial lipase (EL) knockout mice (Ma, K., et al. (2003). Proc Natl Acad Sci USA. 100(5)2748-2753).
Western Blotting Analysis: A representative lot detected purified murine Lcat and Lcat in plasma samples from human A-I transgenic mice with or without scavenger receptor class B type I (SR-BI) knockout, purified mouse LCAT (Lee, J.Y., et al. (2007). J Lipid Res. 48(5)1052-1061).
Analysis Note
Evaluated by Western Blotting in mouse serum.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Lcat in ~2.3 µg of mouse serum.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_032516 |
| UniProt accession no. | P16301 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LCAT(3931) |
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