Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3，100UG，MAB5270-100UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

69 kDa

Immunogen

Highly purified ChAT obtained from porcine brain (Ostermann-Fatif et al., 1990).

Application

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Choline Acetyltransferase Antibody, clone 1.B3.9B3 is validated for use in ELISA, IH, IH(P), WB for the detection of Choline Acetyltransferase.
Western blot

ELISA

Immunohistochemistry: 10-20 μg/mL in paraffin embedded sections {See Ratcliffe et al. 1998}

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Reacts with choline acetyltransferase (ChAT)-positive cells from humans, rats and pigs (Ostermann-Fatif et al., 1992a,b).

Physical form

Format: Purified
Protein A purified
Purified immunoglobulin. Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. Reconstitute to 100 μg/mL with sterile distilled water.

Preparation Note

Maintain lyophilized antibody at 2–8°C in undiluted aliquots for up to 6 months. Maintain reconstituted antibody at -20°C in undiluted aliquots for up to 6 months.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Segment	100
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	1.B3.9B3, monoclonal
species reactivity	human, rat, pig
manufacturer/tradename	Chemicon^®
technique(s)	ELISA: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable
isotype	IgG1
NCBI accession no.	NM_020549.3,NM_020984.2,NM_020985.2,NM_020986.2
UniProt accession no.	P28329
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... CHAT(1103) pig ... Chat(396896) rat ... Chat(290567)

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