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- 规格参数
- 包装参数
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa observed. Refer to Cat. No. 07-442 for observed molecular weight information.
Immunogen
Linear peptide corresponding to Histone H3 trimethylated at Lys9.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-trimethyl Histone H3 (Lys9) Antibody, Alexa Fluor™ 647 Conjugate is validated for use in ICC for the detection of trimethyl Histone H3 (Lys9).
Physical form
Protein A purified
Purified rabbit conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1 % Sodium Azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evalutated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys9) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| concentration | 0.5 mg/mL |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NM_003493 |
| UniProt accession no. | Q16695 |
| shipped in | wet ice |
| target post-translational modification | trimethylation (Lys9) |
| Gene Information | mouse ... H3C1(360198) rat ... H3C1(679994) |
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