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- 包装参数
General description
Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.
~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed).
Immunogen
KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.
Epitope: N-terminus
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABT1493) is also available for immunocytochemistry, immunoprecipitation, and Western blotting applications.
Research Category
Cell Structure
This rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor™ 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1.
Biochem/physiol Actions
This Alexa Fluor™ 488-conjugated polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.
Physical form
Affinity purified.
Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cofilin-1 in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 488 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NP_005498 |
| UniProt accession no. | P23528 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... CFL1(1072) |
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