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General description
The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.
Immunogen
Recombinant human Bub1.
Application
Detect Bub1 with Anti-Bub1 Antibody, clone 14H5 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein.
Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.
Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes human Bub1.
Physical form
Format: Purified
Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 14H5, monoclonal |
| species reactivity | primate, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_004336.2 |
| UniProt accession no. | O43683 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... BUB1(699) |
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