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General description
Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity (excitation maximum: ~380 nm; emission maximum: ~460 nm).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Chymotrypsin
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.
Graf, L., et al. 1987. Biochemistry26, 2616.
Oshima, G. 1984. J. Biochem.95, 1131.
Oshima, G. 1983. J. Biochem.94, 1615.
Suc-Ala-Ala-Pro-Phe-AMC
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Level | 100 |
| assay | ≥98% (HPLC) |
| form | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated,protect from light |
| color | white |
| solubility | DMSO: soluble,acetonitrile: water (1:1): soluble |
| fluorescence | λex ~380 nm,λem ~460 nm |
| shipped in | ambient |
| storage temp. | −20°C |
| SMILES string | N1([C@@H](CCC1)C(=O)NC(=O)[C@@H](Nc3cc4c(cc3)C(=CC(=O)O4)C)Cc2ccccc2)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)O)C)C |
| InChI | 1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1 |
| InChI key | IGKPQFYMWKQDQS-KCXKOMAXSA-N |
| 长度(mm) | |
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