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General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.
Recognizes the ~97 kDa glycosylated α-ENaC protein.
This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).
Immunogen
Human
a synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
Application
Immunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol.18, 138.
Does not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | human, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1 µg/mL) Immunoprecipitation ) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SCNN1A(6337) rat ... Scnn1A(25122) |
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