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General description
The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
~95 kDa
Immunogen
Epitope: pTyr694/699
KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.
Application
Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.
Research Category
Signaling
Apoptosis & Cancer
Research Sub Category
Transcription Factors
Biochem/physiol Actions
Phospho-STAT5A/B (Tyr694/699).
Physical form
Cultured supernatant containing 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
PDGF treated 3T3/NIH cells
Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
Other Notes
Replaces: 05-886
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | A11W, monoclonal |
| species reactivity | rat, chimpanzee, human, mouse, bovine |
| technique(s) | western blot: suitable |
| isotype | IgG |
| suitability | not suitable for immunoprecipitation |
| NCBI accession no. | NM_003152,NM_012448 |
| UniProt accession no. | P42229 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pTyr694/pTyr699) |
| Gene Information | bovine ... Stat5A(282375) |
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