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General description
Amyloid-beta A4 precursor protein-binding family B member 1 (UniProt: Q9QXJ1; also known as Protein FE65) is encoded by the Apbb1 (also known as FE65) gene (Gene ID: 11785) in murine species. FF65 is an adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. It is selectively expressed in the brain with high expression in regions affected in Alzheimer s disease. FE65 is phosphorylated at Threonine 579 by GSK-3 beta, which potentiates gamma and beta secretase-mediated APP processing and beta-amyloid liberation. This phosphorylation is also reported enhance FE65/APP complex formation. FE65 also plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. FE65 may act by specifically recognizing and binding histone H2AX phosphorylated on Tyrosine 142 (H2AXY142ph) at double-strand breaks, recruiting other pro-apoptosis factors such as MAPK8/JNK1. FE65 contains an N-terminal WW domain (aa 253-285) and two C-terminal phosphotyrosine interaction domains (aa 370-509, PID1 and aa 542-699, PID2). (Ref.: Lee, YS et al. (2017). Sci. Rep. 7(1):12456).
~97 kDa observed; 77.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: cytoplasmic domain
GST-tgged recombinant fragment corresponding to 40 amino acids from the N-terminal half of murine Protein FE65.
Application
Anti-FE65, clone 3H6, Cat. No. 05-758-I, is a highly specific mouse monoclonal antibody that targets FE65 and has been tested for use in Western Blotting.
Research Category
Neuroscience
Western Blotting Analysis: A 1:500 dilution from a representative lot detected FE65 in Tissue extracts of wild type (WT), Fe65 KO, Fe65L1 KO, and Fe65/Fe65L1 DKO mouse brains. (Courtesy of Univ. Prof. Dr. (PhD) Stefan Kins, Technical University of Kaiserslautern, Kaiserslautern, Germany).
Biochem/physiol Actions
Clone 3H6 specifically detects Protein FE65 in murine and human cells.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotype Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b .
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3H6, monoclonal |
| species reactivity | mouse, human |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001240814.1 |
| UniProt accession no. | Q9QXJ1 |
| target post-translational modification | unmodified |
| Gene Information | human ... RACGAP1(29127) mouse ... Apbb1(11785) |
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