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- 规格参数
- 包装参数
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
This PhosphoDetect
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
Application
Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)
Physical form
In PBS.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HEK293 cells
Other Notes
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Level | 200 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | mouse, human, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer232) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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