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General description
Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10
Varies
Immunogen
Epitope: phosphotyrosine
Phosphotyrosine conjugated to carrier protein
Application
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
Biochem/physiol Actions
Recognizes proteins containing phosphotyrosine residues, regardless of origin.
Physical form
100 μL of protein A purified mouse IgG2b in PBS containing preservative and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.
Protein A purified
Preparation Note
6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | peroxidase conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | PY20, monoclonal |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Upstate® |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG2b |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PID1(55022) |
| 长度(mm) | |
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| 重量(kg) |
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NNALGENE 180透明塑料管，透明，柔软，多用，经济，内径 x 外径x壁厚，1/4 x 1/2 x 1/8，英寸，每箱数量50，50/箱，8000-0080，Thermofisher，赛默飞世尔
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