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General description
5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.
This antibody detects 5-hydroxymethylcytosine.
Immunogen
BSA-conjugated 5-hydroxymethylcytosine.
Epitope: 5-hydroxymethylcytosine
Application
Detect 5-hydroxymethylcytosine (5hmC) with Anti-5-hydroxymethylcytosine (5hmC) Antibody, clone HMC 31 (Mouse Monoclonal Antibody), that has been shown to work in DB, MeDIP, ELISA.
ELISA Analysis: A representative lot detected 5-hydroxymethylcytosine in ELISA.
Methylated DNA Immunoprecipitation (MeDIP) Analysis: A representative lot immunoprecipitated 5-hydroxymethylcytosine in ChIP.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Biochem/physiol Actions
This antibody recognizes the 5-hydroxymethylcytosine of 5-Hydroxymethylcytosine DNA.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Unmodified and modified cytosine DNA.
Evaluated by Dot Blot in unmodified and modified cytosine DNA.
Dot Blot Analysis: 0.1 µg/mL of this antibody detected 5-hydroxymethylcytosine in 10 ng and 50 ng of unmodified and modified cytosine DNA.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | HMC 31, monoclonal |
| species reactivity (predicted by homology) | all |
| technique(s) | ELISA: suitable,dot blot: suitable,methylated DNA immunoprecipitation (MeDIP): suitable |
| isotype | IgG1κ |
| shipped in | wet ice |
| target post-translational modification | unmodified |
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