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General description
PADI4 (Protein-arginine deiminase type-4) is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalysis of the deimination of the arginine residues. PADI4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response.
~74 kDa
Immunogen
Epitope: C-terminus
KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of PADI4.
Application
Anti-PADI4 Antibody, C-terminus is an antibody against PADI4 for use in WB, ELISA.
ELISA: A 1:1000 dilution of a previous lot was used on ELISA.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Biochem/physiol Actions
The antibody recognizes PADI4 at and around the C-terminus.
Physical form
Format: Purified
Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Preparation Note
Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC
Analysis Note
Control
HEK293 cell lysate
Evaluated by western blot on HEK293 cell lysate.
Western Blotting Analysis: 1:25-1:500 dilution of this antibody detected PADI4 in HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | saturated ammonium sulfate (SAS) precipitated |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,western blot: suitable |
| NCBI accession no. | NP_036519 |
| UniProt accession no. | Q9UM07 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PADI4(23569) |
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