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General description
Chromobox protein homolog 1 (UniProt: P83916; also known as HP1Hsbeta, heterochromatin protein 1 homolog beta, heterochromatin protein p25, M31, Modifier 1 protein, p25 beta) is encoded by the CBX1 (also known as CBX) gene (Gene ID:10951) in human. Chromobox protein homolog 1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It is essential for formation of and gene repression in heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. The human HP1 homolog CBX1 preferentially recognize H3K9me3 (Peptide binding specificity Kd = 5 µM). However, upon substitution of Threonine 6 to Alanine reduces its affinity significantly (Kd = 45 mM). (Ref.: Kaustov, L., et al. (2011). J. Biol. Chem. 286(1): 521-529).
~23 kDa observed; 21.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant protein fragment corresponding to 60 amino acids from the internal region of human Chromobox protein homolog 1 (CBX1).
Application
Anti-CBX1 (HP1 beta) antibody, clone 5E9.1, Cat. No. MABE664, is a highly specific mouse monoclonal antibody that targets CBX1 and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
Clone 5E9.1 specifically detects human Chromobox protein homolog 1 (CBX1). It targets an epitope with in 60 amino acids from the internal region.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CBX1 (HP1 beta) in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 5E9.1, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001120700 |
| UniProt accession no. | P83916 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... CBX1(10951) |
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