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General description
RING1 belongs to the RING finger family, members of which are characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain.
~42 kDa
Immunogen
GST-tagged recombinant protein corresponding to RING1.
Application
Immunocytochemistry: Representative lot data.
A 1:500 dilution of a previous lot was used in immunocytochemistry on NIH/3T3 and A431 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-RING1 Antibody, clone 4D6 (Mouse Monoclonal Antibody) validated in WB, ICC to detect RING1 also known as Polycomb complex protein RING1.
Biochem/physiol Actions
This antibody detects RING1.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
HEK293 cell lysate
Evaluated by western blot on HEK293 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected RING1 in HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4D6, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_002922.2 |
| UniProt accession no. | Q06587 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RING1(6015) |
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¥ 1683.78
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¥ 1302.89
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¥ 2068.25
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¥ 1653.62
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¥ 20380.83
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¥ 2307.00
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