Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli,50UG,PF126-50UGCN,Sigma

销售价: ¥ 4873.60 / 件
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订货号 0BV6272
品牌型号 Sigma PF126-50UGCN
货期 询货期
最小订货量 1件
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  • 商品介绍
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产品介绍 Product Description

General description

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.


Application

Neutralization (see biological activity)


Biochem/physiol Actions

EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.


Preparation Note

Following initial thaw, aliquot and freeze (-20°C).


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
biological sourcehuman
Quality Segment100
recombinantexpressed in E. coli
assay≥95% (SDS-PAGE)
formliquid
species reactivityhuman
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
dilution (Neutralization (see biological activity))
shipped inwet ice
storage temp.−20°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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