Anti-Brd4 Antibody, clone 1F11，100UG，MABE918，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.
~152 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: a.a. 785-1055 domain
Recombinant protein corresponding to the a.a. 785-1055 domain of human Brd4.

Application

Anti-Brd4, clone 1F11 is an antibody against Brd4 for use in WB, ChIP.
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Brd4 using breast cancer cells MCF-7 (Data courtesy of Cheng-Ming Chiang, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Brd4 in 16 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	1F11, monoclonal
species reactivity	human
technique(s)	ChIP: suitable,western blot: suitable
isotype	IgG1κ
NCBI accession no.	NP_055114
UniProt accession no.	O60885
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... BRD4(23476)

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