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General description
NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
Biochem/physiol Actions
Primary Target
NOS
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
Other Notes
Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
| Quality Level | 100 |
| description | RTECS - XF8045000 |
| assay | ≥98% (TLC) |
| form | crystalline solid |
| potency | 3-4 μM IC50 |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated (hygroscopic),protect from light |
| color | yellow |
| solubility | methanol: soluble,water: soluble |
| shipped in | ambient |
| storage temp. | 2-8°C |
| SMILES string | [Cl-].[Cl-].[n+]2([n](nc(n2)c8ccccc8)c3c(cc(cc3)c4cc(c(cc4)[n]5[n+](nc(n5)c7ccccc7)c6ccc(cc6)[N+](=O)[O-])OC)OC)c1ccc(cc1)[N+](=O)[O-] |
| InChI | 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2 |
| InChI key | FSVCQIDHPKZJSO-UHFFFAOYSA-L |
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