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General description
Synaptophysin (UniProt: P07825; also known as Major synaptic vesicle protein p38) is encoded by the Syp gene (Gene ID: 24804) in rat. Synaptophysin belongs to the synaptophysin/synaptobrevin family of proteins. It is one of the major glycoproteins present in presynaptic vesicles and in various neuroendocrine cells of both neuronal and epithelial phenotype. Its structure consists of a hexameric protein of four transmembrane spans with a short cytoplasmic amino and a long carboxy-terminal tail. Both amino- and carboxy-terminals are exposed on the cytoplasmic surface of the synaptic vesicle membrane. C-terminal domain of Snaptophysin is selectively required for the endocytosis during, but not after, cessation of sustained synaptic transmission. Synaptophysin is involved in organizing membrane components may regulate endocytosis to ensure vesicle availability following sustained neuronal activity. It is also involved in the regulation of short-term and long-term synaptic plasticity. Syp / neurons display functional deficiencies and slower recovery of the recycling synaptic vesicle pool. (Ref.: Kwon, SE and Chapman, ER (2011). Neuron. 70(5): 847 854).
~40 kDa observed; 33.31 kDa calculated. Glycosylated form displays higher molecular weight (~38 kDa). Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant fragment corrsponding to 86 amino acids from the C-terminal region of rat synaptophysin.
Application
Anti-Synaptophysin Antibody, clone 10F6.1, Cat. No. MABN1193, is a highly specific monoclonal antibody that targets Synaptophysin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in human cerebellum, human cerebral cortex, and human pancreatic tissues.
Research Category
Neuroscience
Biochem/physiol Actions
Clone 10F6.1 detects synaptophysin in rat, human, and mouse brain. It targets a sequence within amino acids 222-307 in rat synaptophysin.
Physical form
Format: Purified
Protein G purified
Purified monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: MAB368
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10F6.1, monoclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_036796 |
| UniProt accession no. | P07825 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... SYP(6855) rat ... Syp(24804) |
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