Anti-Ryanodine Receptor 1 Antibody，100UL，AB9078，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

Immunogen

Synthetic peptide from the variant TM region of human Ryanodine Receptor 1.

Application

Anti-Ryanodine Receptor 1 Antibody detects level of Ryanodine Receptor 1 & has been published & validated for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Ryanodine Receptor 1.

Analysis Note

Control
Western blot = adult mouse skeletal muscle (adult mouse cardiac muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

技术参数 Specifications

biological source	rabbit
Quality Level	100
conjugate	unconjugated
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse, rat
manufacturer/tradename	Chemicon^®
technique(s)	immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable
NCBI accession no.	NM_000540.2,NM_001042723.1
UniProt accession no.	P21817
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	human ... RYR1(6261)

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