- 商品介绍
- 规格参数
- 包装参数
General description
The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
Application
This rat monoclonal Anti-Ly-6G Antibody (mouse), APC, clone RB6-8C5 , Cat. No. MABF1477 is validated for use in Flow Cytometry, for the detection of Ly-6G.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | Allophycocyanin conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | RB6-8C5, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2bκ |
| web metadata keyword.default | Gr-1 specific antibody for flow cytometry,Ly-6G labeling antibody for macrophage research,RB6-8C5 APC conjugated antibody,RB6-8C5 antibody APC mouse Ly-6G,RB6-8C5 flow cytometry reagent,Sigma-Aldrich RB6-8C5 Gr-1 antibody,anti-Ly-6G APC antibody flow cytometry,anti-Ly-6G antibody for myeloid lineage studies,anti-Ly-6G antibody,anti-Ly-6G clone RB6-8C5 for cell adhesion studies,flow cytometry grade RB6-8C5 antibody,high purity RB6-8C5 antibody for research,mouse Ly-6G antibody clone RB6-8C5,mouse anti-Gr-1 antibody |
| UniProt accession no. | P35461 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ly6G(546644) |
| 长度(mm) | |
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