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General description
Uracil-DNA glycosylase (EC 3.2.2.27; UniProt P13051; also known as UDG) is encoded by the UNG (also known as DGU, HIGM4, HIGM5, UNG1, UNG2, UNG15) gene (Gene ID 7374) in human. Uracil-DNA glycosylases catalyze the release of uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway that plays an important role in mutagenesis prevention. Uracil bases in DNA can result from cytosine deamination or misincorporation of dUMP instead of dTTP during DNA synthesis. Alternative promoter usage and splicing of UNG gene lead to two different isoforms, the mitochondrial UNG1 and the nuclear UNG2. The mammalian shelterin complex contains the six proteins (TRF1, TRF2, RAP1, TIN2, POT1, and TPP1), the complex occupies the end of the telomeres and protect telomeres against inappropriate DNA repair when telomeres are not being lengthened. UNG deficiency in primary mouse hematopoietic cells is shown to affect POT1 (Protector of Telomeres 1) recognition of the telomere repeats due to increased uracil incorporation in telomeres TTAGGG repeats, which in turn leads to abnormal telomere lengthening.
~34 kDa observed (33.9 kDa/UNG1 & 34.6 kDa/UNG2 calculated). Immunogen sequence should allow to detect both isoform 1 and 2. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Near C-terminus
Linear peptide corresponding to human Uracil-DNA glycosylase near the C-terminus.
Application
Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1, Cat. No. MABE354, is a highly specific mouse monoclonal antibody, that targets UNG and has been tested in Western Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Uracil-DNA glycosylase in human skeletal muscle and human cardiac tissues.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Biochem/physiol Actions
Expected to react with both spliced isoforms (UNG1 & UNG2) of human Uracil-DNA glycosylase.
Physical form
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Uracil-DNA glycosylase in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 8G10.1, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_003353 |
| UniProt accession no. | P13051 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UNG(7374) |
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¥ 459.57
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¥ 1048.84
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¥ 1125.70
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¥ 3317.36
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