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General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
~17 kDa
Immunogen
Epitope: Phospho Ser10
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.
Application
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histones
Biochem/physiol Actions
Broad species cross-reactivity is expected, based on sequence homology.
This antibody specifically recognizes Histone H3 phosphorylated at Ser10.
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa Acid extract lysate
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | CMA312, monoclonal |
| species reactivity | human, vertebrates |
| manufacturer/tradename | Upstate® |
| technique(s) | ChIP: suitable,ELISA: suitable,immunocytochemistry: suitable,multiplexing: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_003484.1 |
| UniProt accession no. | P84243 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pSer10) |
| Gene Information | human ... H3C1(8350) |
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