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- 规格参数
- 包装参数
General description
Cullin-3 (UniProt Q13618) is encoded by the CUL3 (also known as PHA2E, KIAA0617) gene (Gene ID 8452) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Cullin-3 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). Cullin-3-based CRLs rely on Cullin-3 interaction with bric-a-brac/tramtrack/broad-complex (BTB) domain-containing proteins for targets recruitment.
~88 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: C-terminal
GST-tagged recombinant protein corresponding to the C-terminal of human Cullin-3.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Cullin-3 Antibody, clone 3A8.1 is validated for use in Western Blotting for the detection of Cullin-3.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse neuroblastoma Neuro-2a (N2a) cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-3 in 10 µg of mouse neuroblastoma Neuro-2a (N2a) cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3A8.1, monoclonal |
| species reactivity | monkey, mouse |
| species reactivity (predicted by homology) | human (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_003581 |
| UniProt accession no. | Q13618 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CUL3(8452) |
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¥ 4173.75
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¥ 5725.45
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¥ 3335.66
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¥ 2617.87
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¥ 1586.69
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