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Anti-NRF-1 Antibody, clone R157.1.3H3，100UG，MABE995，Sigma

销售价： ¥ 3637.90 / 件

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订货号： 0BV5051

品牌型号： Sigma MABE995

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最小订货量： 1件

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产品介绍 Product Description

General description

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
~58 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Recombinant protein corresponding to human NRF-1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected NRF-1 in 10 µg of SH-SY5Y and HeLa cell lysates.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NRF-1 chromatin DNA binding activity using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
ChIP-seq Analysis: A representative lot detected NRF-1 DNA binding motifs by ChIP-seq analysis using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF-1 in 10 µg of Raji cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Segment	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	R157.1.3H3, monoclonal
species reactivity	human
technique(s)	ChIP: suitable (ChIP-seq),western blot: suitable
isotype	IgG2aκ
NCBI accession no.	NP_001035199
UniProt accession no.	Q16656
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... NRF1(4899)

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